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type 1 collagenase bp2649  (Fisher Bioreagents)

 
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    Structured Review

    Fisher Bioreagents type 1 collagenase bp2649
    Type 1 Collagenase Bp2649, supplied by Fisher Bioreagents, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/type+1+collagenase+bp2649/type+1+collagenase+bp2649/10__1089_slash_ten__tea__2022__0185-43-45-49
    Average 90 stars, based on 1 article reviews
    type 1 collagenase bp2649 - by Bioz Stars, 2026-10
    90/100 stars

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    Modification:

    Article Title: Exosome Cell Origin Affects <i>In Vitro</i> Markers of Tendon Repair in Ovine Macrophages and Tenocytes
    Article Snippet: .. These tissue sections were digested in 35 mL of 1:1 Dulbecco’s Modified Eagle’s Medium with Nutrient Mixture F-12 Ham with 15 mM HEPES and sodium bicarbonate (DMEM:F12, D6421; Sigma-Aldrich) with 200 units penicillin, 0.2mg/mL streptomycin, and 0.5mg/mL amphotericin B (Abx, A5955; Sigma-Aldrich) and 1mg/mL of type 1 collagenase (BP2649; Fisher Bioreagents) for 24 h at 37 C with 5% carbon dioxide under constant agitation as a modification of traditional enzymatic cell isolation.22–24 This digesta was then filtered at 100mm (Cell Strainer, Z742101; Sigma-Aldrich), and the flow through was diluted to 50 mL with Dulbecco’s phosphate buffered saline (PBS; D1408; Sigma-Aldrich), centrifuged at 400 g for 10 min, and the cell pellet was resuspended in and washed twice in 10 mL of PBS. .. The resulting cell pellet was resuspended in DMEM:F12 with 10% Fetal Bovine Serum (97068-085, Avantor) and Abx (complete DMEM [CD]) and grown to confluence in a 75 cm2 culture flask (156499; Thermo Fisher Scientific) before passage.

    Isolation:

    Article Title: Exosome Cell Origin Affects <i>In Vitro</i> Markers of Tendon Repair in Ovine Macrophages and Tenocytes
    Article Snippet: .. These tissue sections were digested in 35 mL of 1:1 Dulbecco’s Modified Eagle’s Medium with Nutrient Mixture F-12 Ham with 15 mM HEPES and sodium bicarbonate (DMEM:F12, D6421; Sigma-Aldrich) with 200 units penicillin, 0.2mg/mL streptomycin, and 0.5mg/mL amphotericin B (Abx, A5955; Sigma-Aldrich) and 1mg/mL of type 1 collagenase (BP2649; Fisher Bioreagents) for 24 h at 37 C with 5% carbon dioxide under constant agitation as a modification of traditional enzymatic cell isolation.22–24 This digesta was then filtered at 100mm (Cell Strainer, Z742101; Sigma-Aldrich), and the flow through was diluted to 50 mL with Dulbecco’s phosphate buffered saline (PBS; D1408; Sigma-Aldrich), centrifuged at 400 g for 10 min, and the cell pellet was resuspended in and washed twice in 10 mL of PBS. .. The resulting cell pellet was resuspended in DMEM:F12 with 10% Fetal Bovine Serum (97068-085, Avantor) and Abx (complete DMEM [CD]) and grown to confluence in a 75 cm2 culture flask (156499; Thermo Fisher Scientific) before passage.

    Saline:

    Article Title: Exosome Cell Origin Affects <i>In Vitro</i> Markers of Tendon Repair in Ovine Macrophages and Tenocytes
    Article Snippet: .. These tissue sections were digested in 35 mL of 1:1 Dulbecco’s Modified Eagle’s Medium with Nutrient Mixture F-12 Ham with 15 mM HEPES and sodium bicarbonate (DMEM:F12, D6421; Sigma-Aldrich) with 200 units penicillin, 0.2mg/mL streptomycin, and 0.5mg/mL amphotericin B (Abx, A5955; Sigma-Aldrich) and 1mg/mL of type 1 collagenase (BP2649; Fisher Bioreagents) for 24 h at 37 C with 5% carbon dioxide under constant agitation as a modification of traditional enzymatic cell isolation.22–24 This digesta was then filtered at 100mm (Cell Strainer, Z742101; Sigma-Aldrich), and the flow through was diluted to 50 mL with Dulbecco’s phosphate buffered saline (PBS; D1408; Sigma-Aldrich), centrifuged at 400 g for 10 min, and the cell pellet was resuspended in and washed twice in 10 mL of PBS. .. The resulting cell pellet was resuspended in DMEM:F12 with 10% Fetal Bovine Serum (97068-085, Avantor) and Abx (complete DMEM [CD]) and grown to confluence in a 75 cm2 culture flask (156499; Thermo Fisher Scientific) before passage.



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    type 1 collagenase bp2649  (Fisher Bioreagents)
    90
    Fisher Bioreagents type 1 collagenase bp2649
    Type 1 Collagenase Bp2649, supplied by Fisher Bioreagents, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/type+1+collagenase+bp2649/type+1+collagenase+bp2649/10__1089_slash_ten__tea__2022__0185-43-45-49
    Average 90 stars, based on 1 article reviews
    type 1 collagenase bp2649 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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